Xiaoming Jin, Ph.D.
Associate Professor of Anatomy & Cell Biology
PhD, West Virginia University (2002)
Postdoctoral fellowship, Stanford University (2008)
Mechanisms of functional recovery and epileptogenesis in traumatic brain injury.
Our lab is interested in (1) organization and plasticity of cortical circuits, and (2) epileptogenesis following traumatic brain injury. These topics are investigated with a variety of techniques at molecular, cellular and circuit levels, including laser scanning photostimulation combined with whole cell patch clamp recording, organotypic brain slice culture, gene gun transfection, time lapse confocal imaging, and molecular biological techniques. The long-term goals of the lab are to develop therapeutic strategies for the prevention and treatment of posttraumatic epilepsy, for improvement of functional recovery in patients with traumatic brain injury.
Current Studies Include:
A) Understanding the roles and regulation of axonal sprouting in posttraumatic epilepsy. Axonal sprouting is considered one of the major pathological changes in different types of epilepsy, including temporal lobe and posttraumatic epilepsies. This regenerative process also provides a structural basis for functional recovery of the cerebral cortex following various brain damages such as stroke and brain trauma. It is critical to find a way to preserve normal axonal regeneration without maladaptive connectivity that contributes to epileptic seizures after injuries. Using organotypic slice culture and acute brain slice preparation, we are currently studying how axonal sprouting occurs in cortical pyramidal neurons following injury, and if it plays an important role in posttraumatic epileptogenesis.
B) Anatomy and neurophysiology of the cortical circuits. Understanding how different types of cortical neurons are wired into functional circuits is fundamental to deciphering cortical functions and disorders. We use a combination of glutamate uncaging and whole cell patch clamp recording techniques to study the organization of neural circuits in the neocortex, particularly the long-range corticocortical connections between somatosensory and motor cortices.
Jin X, P. Mathers, G. Szabo, Z. Katarova and A. Agmon (2001). Vertical bias in dendritic trees of non-pyramidal neocortical neurons expressing GAD67-GFP in vitro. Cereb Cortex. 2001 Jul; 11(7):666-78.
Jin X, Hang Hu, Peter H. Mathers, and Ariel Agmon (2003). Brain-derived neurotrophic factor mediates activity-dependent dendritic growth in nonpyramidal neocortical interneurons in developing organotypic cultures. Journal of Neuroscience, 2003 Jul; 23 (13):5662-5673.
Jin X, John R. Huguenard, and David A. Prince (2005). Impaired Cl- Extrusion in Layer V Pyramidal Neurons of Chronically Injured Epileptogenic Neocortex. Journal of Neurophysiology, 2005 Apr;93(4):2117-26.
Jin X, David A. Prince and John R. Huguenard (2006). Enhanced Excitatory Synaptic Connectivity in Layer V Pyramidal Neurons of Chronically Injured Epileptogenic Neocortex in Rats. Journal of Neuroscience, 2006 May; 26(18):4891 - 4900.
Sanjay S. Kumar*, Jin X*, Paul S. Buckmaster, and John R. Huguenard (2007). Recurrent circuits in layer II of medial entorhinal cortex in a model of temporal lobe epilepsy. Journal of Neuroscience, 2007 Feb 7;27(6):1239-46. (* co-first author).
Prince DA, Parada I, Scalise K, Graber K, Jin X, Shen F. Epilepsy following cortical injury: cellular and molecular mechanisms as targets for potential prophylaxis. Epilepsia. 2009 Feb;50 Suppl 2:30-40.
Yunxiang Chu*, Jin X*, Isabel Parada, Alexei Pesic, Beth Stevens, Ben Barres, David A. Prince. Enhanced synaptic connectivity and epilepsy in C1q knockout mice. PNAS. 2010 Apr 27;107(17):7975-80. (* co-first author).
Jin X, John R. Huguenard, David A. Prince. Reorganization of Inhibitory Synaptic Circuits in Rodent Chronically Injured Epileptogenic Neocortex. Cerebral Cortex. 2011 May;21(5):1094-104.
Joel M. Brittain, Djane B. Duarte, Sarah M. Wilson, Yuying Wang, Weiguo Zhu, Carrie Ballard, May Khanna, Tatiana Brustovetsky, Brian S. Schmutzler, Wenhui Xiong, Matthew S. Ripsch, Nicole M. Ashpole, Andy Hudmon, Cynthia M. Hingtgen, Nikolay Brustovetsky, Jin X, Michael R. Vasko, Jill C. Fehrenbacher, Joyce H. Hurley, Fletcher A. White, and Rajesh Khanna. Suppression of inflammatory and neuropathic pain by uncoupling CRMP-2 from the presynaptic Ca2+ channel complex. Nature Medicine. 2011 Jun 5; 17(7):822-9.
Wenhui Xiong, Xingjie Ping, Jianhua Gao, Jin X. Preparing undercut model of posttraumatic epileptogenesis in rodents. J Vis Exp. 2011 Sep 15;(55).
Sarah M. Wilson, Wenhui Xiong, Jessica D. Head, Joel M. Brittain, †Jin X, †Rajesh Khanna. RMP2 mediated neurite outgrowth and tubulin polymerization are blocked by subtherapeutic levels of the anticonvulsant Lacosamide. Neuroscience. 2012. † Co-corresponding author.
Wenhui Xiong, Jin X (2012). Optogenetic field potential recording. Journal of Neuroscience Methods. 2012 Sep 30; 210(2):119-24.
Jiangping Wang; Keming Ren; Zhongxia Li; Congying Zhao; Guoxia Shen; Jin X; Kewen Jiang. Opening astrocytic mitochondrial ATP-sensitive potassium channels upregulates electrical coupling between hippocampal astrocytes in rat brain slices. PlosOne. In revision.